期刊论文

Antitumor effect and mechanism of Clerodendron Bungei extract on H22 tumor-bearing mice

中文

中国临床药理学杂志

1001-6821

2019

35

14

1459-1462

10.13699/j.cnki.1001-6821.2019.14.013

国家中医药管理重大专项基金资助项目(国中医药人教函[2012]228号) 湖南中医药大学校级科研基金资助项目(2017XJJJ21).

本校为第一机构

目的研究臭牡丹提取物对H22实体移植瘤的抑制作用及其机制。方法皮下接种H22瘤株建立小鼠H22实体移植瘤模型。按照体重将荷瘤鼠随机分为5组:模型组、对照组和低、中、高3个剂量实验组,每组12只。模型组给予0. 9%NaCl灌胃;对照组给予5 -氟尿嘧啶(5-Fu) 25 mg·kg~(-1)腹腔注射;低、中、高3个剂量实验组分别灌胃臭牡丹提取物0. 55,1. 09,2. 18 g·kg~(-1),1次/天,连续14 d。称重法检测小鼠肿瘤重量;以流式细胞术检测外周血CD4+和CD8+ T淋巴细胞;以免疫印迹法检测磷脂酰肌醇3-激酶(PI3K)和磷酸蛋白激酶(p-Akt)蛋白的表达水平。结果模型组、对照组和低、中、高3个剂量实验组肿瘤重量分别为(4. 13± ...

Objective To study the inhibitory effect of Clerodendron Bungei extract on H22 solid xenograft and its mechanism. Methods A mouse H22 solid xenograft model was established by subcutaneous inoculation of H22 tumor strain. The tumor-bearing mice were randomly divided into five groups: model group,control group,and experimental-L,experimental-M and experimental-H groups,with 12 mice in each group. The model group was given normal saline; control group was given 5-fluorouracil (5-Fu) 25 mg·kg~(-1) intraperitoneally; experimental-L,experimental-M a...