期刊论文

Inhibitory effect and mechanism of Clerodendrum bungei-containing serum on invasion and migration of human hepato cellular carcinoma cell line MHCC97-H

中文

中国临床药理学杂志

1001-6821

2019

35

21

2691-2694

10.13699/j.cnki.1001-6821.2019.21.012

国家中医药管理局重大基金资助项目(国中医药人教函[2012]228号) 湖南中医药大学校级科研基金资助项目(2017XJJJ21)。

本校为第一机构

目的探讨臭牡丹含药血清对人肝癌细胞( MHCC97-H细胞)迁移与侵袭能力的影响及其机制。方法按照体重将SD大鼠随机分成4组:模型组和低、中、高3个剂量实验组,每组5只。低、中、高3个剂量实验组给予臭牡丹提取物0.55, 1.09, 2.18 g·kg~(-1);模型组给予0.9%NaCl,每日灌胃2次,连续3 d。腹主动脉取血后分离血清,制备各组含药血清,体外培养MHCC97-H细胞。 Transwell实验检测细胞的迁移能力;免疫印迹法检测磷脂酰肌醇3激酶/蛋白激酶B( PI3K/Akt)信号通路中的磷酸Akt ( p-Akt)和PI3K相关蛋白表达的影响。结果模型组和低、中、高3个剂量实验组的迁移细胞数分别为( 256.0 ± 28.6), ( 181.0 ± 15.7), ( 1...

Objective To investigate the effects of serum containing Clerodendrum bungei on the migration and invasion of human hepatoma cellular carcinoma MHCC97-H cells, and to study its preliminary mechanism. Methods SD rats were randomly divided into 4 groups according to body weight: model group and experimental-L,experimental-M, experimental-H groups,each group had 5 rats. The experimental-L, experimental-M,experimental-H groups were given 0.55,1.09,2.18 g·kg~(-1) of the extract of Clerodendrum bungei; model group was given normal saline; twice a da...