期刊论文

Target genes for advanced hepatocellular carcinoma based on messenger RNA and microRNA chips

中文

中国临床药理学杂志

1001-6821

2020

36

13

1837-1841

10.13699/j.cnki.1001-6821.2020.13.017

国家自然科学基金资助项目(81873303) 湖南省自然基金青年基金资助项目(2016JJ6113) 湖南中医药大学中医学一流学科开放基金资助项目(2018ZYX51)。

本校为其他机构

目的筛选进展期原发性肝癌(HCC)相关靶基因。方法从Gene Expressed Omnibus (GEO)数据库下载信使核糖核酸(mRNA)和微型核糖核酸(miRNA)表达谱。通过limma函数包分析得出差异表达mRNA、miRNA。基于FunRich软件对差异表达的miRNA进行转录因子富集分析。利用TargetScan筛选由差异表达miRNA调节的靶基因,并用Cytoscape软件构建miRNA - mRNA网络图。基于R软件对miRNA - mRNA进行联合基因本体(Gene Ontology, GO) 、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)富集进行分析。结果从进展期HCC样品中筛选出2382个差异表达mRNAs、213个差异表达miRNAs,对应的转录因子中以E...

Objective To screen target genes associated with advanced hepatocellular carcinoma (HCC). Methods Messenger RNA (mRNA) and MicroRNA (miRNA) expression profiles were downloaded from the Gene Expressed Omnibus (GEO) database. Differentially expressed mRNA and miRNA were obtained by limma package analysis. Differentially expressed mirnas were analyzed for transcription factor enrichment using FunRich software. Target genes regulated by differentially expressed mirnas were screened by TargetScan,the mirna - mrna network diagram was constructed by Cytoscape software,and mirna - mrna was enriched by...