期刊论文

Protection mechanism of Jia Wei Dan Shen Yin combined with microRNA-21 on rat myocardial cell

中文

中国临床药理学杂志

1001-6821

2020

36

1

61-64

10.13699/j.cnki.1001-6821.2020.01.017

国家自然科学基金青年科学基金资助项目(81503565) 湖南省教育厅优秀青年基金资助项目(16B198) 中医内科学省部共建教育部重点实验室开放基金重点基金资助项目(ZYFT201507) 湖南省教育厅基金资助项目(14K073)。

本校为第一机构

目的 研究加味丹参饮(JWDSY)对心肌细胞的保护作用和及其可能机制.方法 将心肌细胞分为 4 组:空白组、模型组、微小核糖核酸 21 组(microRNA-21组)和JWDSY组.空白组不做任何处理;模型组将心肌细胞进行缺氧复氧交替培养;microRNA-21组将慢病毒载体转染入H9C2缺氧复氧模型心肌细胞后造模,加入空白血清共孵育;JWDSY组将慢病毒载体转染入H9C2心肌细胞后造模,加入JWDSY含药血清共孵育.用试剂盒法分别检测乳酸脱氢酶的释放量(LDH)和心肌细胞的存活率,用荧光定量PCR法检测微小核糖核酸21(microRNA-21)基因相对表达量.结果 空白组、模型组、microRNA-21组和JWDSY 组的 LDH 释放量分别为(373.97 ± 17.73...

Objective To investigate the protective effect and possible mechanism of Jia Wei Dan Shen Yin(JWDSY) on myocardial cell. Methods Myocardial cells were divided into four groups: blank group, model group, microRNA-21 group and JWDSY group. The cells in the blank group did not do any treatment;myocardial cells in model group were alternately cultured with hypoxia and reoxygenation;microRNA-21 group is treated with virus vector transfection into cardiomyocytes H9C2 and incubation with blank serum;JWDSY group is treat with virus vector transfection ...