期刊论文

Effects of metformin combined with donepezil on the activation of hippocampal microglia and cognitive function in diabetic rats

中文

中国临床药理学杂志

1001-6821

2021

37

22

3115-3118

10.13699/j.cnki.1001-6821.2021.22.024

湖南省自然科学基金资助项目（2019JJ50345;2020JJ5325）； 湖南省中医药研究院重点基金资助项目（201803）；

本校为第一机构

目的探讨二甲双胍联合多奈哌齐对糖尿病大鼠海马小胶质细胞活化、犬尿氨酸通路和认知功能的影响。方法将血糖值≥11.1 mmol·L~(-1)的Goto-Kakizaki(GK)大鼠随机分为3组:模型组、二甲双胍组、联合组;另选正常Wistar大鼠作为正常组。二甲双胍组以76.5 mg·kg~(-1)二甲双胍干预,联合组给予76.5 mg·kg~(-1)二甲双胍+3 mg·kg~(-1)多奈哌齐干预,正常组和模型组给予等量纯净水,连续4周。以Morris水迷宫法检测大鼠在连续5 d的学习和记忆能力,免疫荧光双标法检测海马吲哚胺2,3双加氧酶(IDO) +离子钙结合衔接分子(Iba -1)蛋白表达(平均阳性表达率),实时荧光定量-PCR法检测大鼠海马犬尿氨酸3-单加氧酶(K...

Objective To explore the effects of metformin combined with donepezil on hippocampal microglia cell viability,kynurenine pathway and cognitive function in diabetic rats.Methods Goto-Kakizaki(GK) rats were randomly divided into three groups with blood glucose level ≥ 11.1 mmol·L~(-1):model group,metformin group,and combination group.Wistar rats were used as normal group.The metformin group was treated with 76.5 mg · kg~(-1) metformin,the combination group was treated with 76.5 mg ·kg~(-1) metformin and 3 mg·kg~(-1) donepezil,the normal grou...