期刊论文

Effect and mechanism of Xuefu Zhuyu Decoction on the senescence of peripheral blood endothelial progenitor cells in myocardial ischemia rats

中文

中国临床药理学杂志

1001-6821

2021

37

14

1832-1835,1839

10.13699/j.cnki.1001-6821.2021.14.015

国家自然科学基金资助项目（81574039）； 湖南省自然科学基金资助项目（2020JJ4471）；

本校为第一机构

目的观察血府逐瘀汤对心肌缺血大鼠外周血内皮祖细胞(EPCs)衰老及抑癌基因p53、沉默信息调节因子(SIRT1)蛋白表达的影响。方法结扎冠状动脉左前降支建立心肌缺血大鼠模型。按照体重将大鼠随机分为7组:正常组、假手术组、模型组、他汀组和低、中、高3个剂量实验组,每组8只。造模成功后第2天开始给药。他汀组腹腔注射阿托伐他汀0.3 mg,低、中、高剂量组灌胃药液3.51,7.02,14.04 g·kg ~(-1),1次/天。于灌胃前和灌胃第3天、第7天,经眼眶内眦静脉丛采血并分离外周血EPCs。以β -半乳糖苷酶染色法检测EPCs衰老状态;以蛋白质印迹法检测EPCs中p53、SIRT1蛋白的表达。结果灌胃第7天,正常组、假手术组、模...

Objective To observe the effects of Xuefu Zhuyu Decoction on the senescence of endothelial progenitor cells (EPCs) and the expression of tumor suppressor p53 and silencing information regulator 1 (SIRT1) in peripheral blood of rats with myocardial ischemia. Methods The left anterior descending coronary artery was ligated to establish a rat model of myocardial ischemia. The rats were randomly divided into 7 groups according to weight: normal group (no treatment), sham operation group (only threading and no ligation),model group (orally administe...