期刊论文

Effect of exosomes from endothelial progenitor cells mediated by Astragaloside Ⅳ on the proliferation and apoptosis of endothelial cells damaged by high glucose

中文

中国临床药理学杂志

1001-6821

2021

37

15

2048-2050

10.13699/j.cnki.1001-6821.2021.15.025

国家自然科学基金青年科学基金资助项目（81904217）； 湖南省自然科学基金青年基金资助项目（2019JJ50460）； 湖南省卫生计生委科研计划基金资助项目（20201388）；

本校为第一机构

目的探讨黄芪甲苷(AS -Ⅳ)介导的内皮祖细胞外泌体(EPCs - Exos)对高糖受损内皮细胞(ECs)增殖和凋亡的影响。方法从足月健康新生儿脐带血中分离、培养单个核细胞,用CD31抗体联合4,6 -二脒基- 2 -苯基吲哚(DAPI)核染鉴定,获得的EPCs用100 mg·L ~(- 1) AS -Ⅳ干预24 h。用超速离心法结合超滤法提取细胞上清液中囊泡结构,用透射电子显微镜进行形态观察,用蛋白质印迹(WB)法检测外泌体表面特征性标志蛋白CD9、CD63、CD81。体外分离、培养人脐静脉内皮细胞(HUVECs),用vWF因子联合DAPI核染鉴定,获得的HUVECs用30 mmol·L ~(- 1)葡萄糖干预120 h,随机分为实验组、对照组并设置正常组(用正常EGM - 2培养...

Objective To investigate the effects of endothelial progenitor cells exosomes (EPCs - Exos) mediated by Astragaloside Ⅳ (AS -Ⅳ) on the proliferation and apoptosis of endothelial cells damaged by high glucose. Methods Mononuclear cells were isolated and cultured from umbilical cord blood of healthy full - term newborns,and identified with CD31 antibody combined with 4,6 - diamidino - 2 - phenylindole (DAPI) nuclear staining. EPCs obtained were used 100 mg·L ~(- 1) AS -Ⅳ intervention for 24 h. The vesicle structure in the cell supernatant was...