期刊论文

Effect and mechanism of nuclear factor - κB inhibitor combined with nuclear factor E2 related factor 2 inhibitor on apoptosis of human colon cancer HCT116 cells

中文

中国临床药理学杂志

1001-6821

2022

38

2

123-126,141

10.13699/j.cnki.1001-6821.2022.02.006

湖南省自然科学基金资助项目（2018JJ2219）；

本校为第一机构

目的探究核因子κB(NF - κB)抑制剂(BAY11 - 7082)联合核因子E2相关因子2(Nrf2)抑制剂(ML385)对人结肠癌细胞HCT116的作用机制。方法体外培养人结肠癌细胞HCT116,将细胞分为4组:空白对照组、NF - κB抑制剂组、Nrf2抑制剂和联合组。空白对照组以RPMI - 1640培养基进行培养,NF - κB抑制剂组以10 μmol · L ~(- 1) BAY 11 - 7082干预,Nrf2抑制剂组以5 μmol·L~(- 1) ML385干预,联合组以10 μmol·L~(- 1) BAY 11 - 7082 + 5 μmol·L~(- 1) ML385干预,干预时间为24 h。MTT法检测人结肠癌细胞HCT116增殖抑制率,以流式细胞术和Hoechst 33342染色法检测细胞凋亡状况,以蛋白质印迹法检测凋亡相关蛋...

Objective To explore the effect of nuclear factor κB (NF - κB) inhibitor (BAY11 - 7082) combined with nuclear factor E2 - related factor 2 (Nrf2) inhibitor (ML385) on the apoptosis of human colon cancer HCT116 cells,and to explore the mechanism of action from the interaction of signal pathways. Methods Human colon cancer HCT116 cells were cultured in vitro. They were divided into 4 groups:Blank control group,NF - κB inhibitor group,Nrf2 inhibitor,and combined group. The blank control group was cultured in RPMI - 1640 medium,and NF - κB inhi...