期刊论文

Screening of neovascularization - related microRNAs in exosomes of mesenchymal stem cells by bioinformatics analysis and the intervention of astragaloside Ⅳ

中文

中国临床药理学杂志

1001-6821

2022

38

1

31-34,39

10.13699/j.cnki.1001-6821.2022.01.008

国家自然科学基金青年基金资助项目（81904217）； 湖南省卫生计生委科研计划课题基金资助项目（20201388）；

本校为第一机构

目的探讨黄芪甲苷(AS -Ⅳ)对生信分析筛选出的人脐带血间充质干细胞(hUCBMSCs)外泌体(Exos)中与血管生成相关微小RNAs(miRNAs)表达的影响。方法通过生信分析筛选hUCBMSCs - Exos中与血管生成高度相关的miRNAs。将hUCBMSCs随机分为实验组和对照组。实验组给予含300 mg·L~(- 1) AS -Ⅳ的DMEM/F12培养基培养24 h,对照组加入等量磷酸盐缓冲溶液培养24 h。用二辛丁酸法检测Exos浓度,用实时荧光聚合酶链反应法检测miRNAs的表达水平。结果实验组和对照组的外泌体浓度分别为(1.22 ± 0.02)和(0.88 ± 0.03) μg·μL ~(- 1),差异有统计学意义(P < 0.01) 。miRNA - 126 - 3p、 miRNA - 21、miRNA - 214 ...

Objective To explore the effect of astragaloside Ⅳ(AS -Ⅳ) on the expression of neovascularization - related miRNAs in the exosomes (Exos) of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) screened by bioinformatics analysis. Methods The miRNAs highly related to neovascularization in hUCBMSCs - Exos was screened by bioinformatics analysis. The hUCBMSCs were randomly divided into experimental and control groups. The experimental group was given DMEM/F12 medium containing 300 mg·L~(- 1) AS - Ⅳ for 24 hours,and the control group w...