期刊论文

Effects of sodium aescinate on expression of neuron Bax and Bcl-2 in intracerebral hemorrhage rats

中文

中国保健营养(中旬刊)

1004-7484

2012

z1

17-18

湖南省教育厅资助项目;湖南省重点学科(中西结合基础)资助

本校为第一机构

目的:研究大鼠脑出血后脑神经元Bax(x蛋白基因)、Bcl-2(B细胞淋巴瘤/白血病-2基因)的表达及七叶皂苷钠的干预作用.方法:采用Ⅶ型胶原酶脑内立体定位注射诱导大鼠脑卒中模型,60只SD 大鼠随机分为正常组、假手术组、模型组、七叶皂苷钠组.选择术后1 d、3 d、7d 3个时间点处死大鼠后取脑,每点5只.采用免疫组织化学SABC 技术,检测出血灶周围脑组织Bax 、Bcl-2蛋白的表达.结果:①模型组血肿周围Bax 、Bcl-2蛋白免疫反应阳性细胞1d 出现,3d 达最大值,7d 仍有表达(P

Objective:To investigate the expression of Bax and Bcl-2 around Hematoma in Intracerebral Hemorrhage(ICH)Rats and the intervention of Sodium Aescinate. Methods:ICH models were prepared with the injection of TypeⅦcollagenase into the cerebrum in rats. Sixty SD rats were randomly divided into normal group, sham-operated(Sham)group, model(Md)group and Sodium Aescinate injection (AS)group. Five rats were sacrificed respectively at 1d, 3d and 7d postoperated. Immunohistochemistry SABC method was performed for examming the expression of Bax and Bcl-...