期刊论文

中文

生物技术通报

1002-5464

2007

4

152-154,168

湖南润孚生物工程有限公司规划项目（No.0629）

本校为其他机构

为了探讨航天与常规诱变技术对MT工程菌株的影响，进行了HNO2、UV、γ-射线、LiCl及γ-射线＋LiCl分别与航天共诱变对MT工程菌株活性的影响试验，并且就航天对MT工程菌株的致死作用及质粒的稳定性进行了研究。结果表明：MT工程菌株经航天处理后，活菌数降低，死亡率为34．59%，航天对MT工程菌株具有一定的致死作用；MT工程菌株经UV、γ-射线、LiCl及γ-射线＋LiCl分别与航天共诱变处理后的生长活性没有改变，MT工程菌株经HN02与航天共诱变处理后的生长速率减慢；在航天处理的作用下，MT工程菌株的质粒不易丢失，能够维持很好的稳定性，丢失率仅为8．26％，而置于地面的MT工程菌株的质粒易丢失，...

In order to probe into the effect of co-mutation by space and ordinary technology on MT engineering strain,a study on the effect of space mutation with HNO2,UV,γ-irradiation,LiCl and γ-inadiation together with LiCl was conducted.The results showed that space mutation would lead 34.59% MT engineering strain to death.The activities didn＇t change when MT engineering strain were mutated by space with UV, γ-irradiation,LiCl and γ-irradiation together with LiCl,but the activities changed when MT engineering strain space was mutated by space with...