期刊论文

中文

肿瘤药学

2095-1264

2011

1

1

33-36

10.3969/j.issn.2095-1264.2011.01.008

湖南省科技计划项目资助,项目编号（2010SK3016）；

本校为第一机构

目的 研究雷公藤甲素对宫颈癌Hele 细胞增殖的抑制作用.方法 采用MTT 方法检测雷公藤甲素对体外培养的宫颈癌Hele 细胞增殖的影响,运用流式细胞术检测细胞周期,RT-PCR 分析细胞周期蛋白B1(cyclin B1)mRNA的表达;Western-blot检测cyclin B1、P34cdc2和磷酸化P34cdc2(phosphorcdc2)蛋白的表达.结果 雷公藤甲素浓度依赖性(0.5~4.0 μg·mL-1)抑制体外培养的宫颈癌Hele细胞,干扰Hele细胞周期,细胞周期阻滞于S期和G2/M期,S期和G2/M期的百分率上升;同时G0/G1细胞百分率降低;RT-PCR结果显示,雷公藤甲素(0.5~4.0 μg·mL-1)显著抑制cyclin B1 mRNA的表达;Western-blot结果显示,雷公藤甲素(0.5~4.0 μg·...

Objective To study the effect of mptohde on proliferation of cervical cancer Hele cells in vitro and its related mechanisms. Method Inhibitory effect of cell proliferation was evaluated by MTF method; Cell cycle distributions were measured by flow cytometric assay; RT-PCR was used to analyze the expression of cyclin B 1 mRNA; Western blotting analysis was used to detect the protein expression of cyclin B1 ,P34cdc2 and phorylation P34cdc2. Results Triptolide （0.5-40 μg-mL-1） suppressed proliferation of cervical cancer Hele cells in a concentr...