Objective Establish the method of preparating and identificating the antiserum against human LOH12CR1 protein. Method We constructed GST-LOH12CR1 fi~sion protein exp~ssion sys- tem, induced high expression of the fusion protein, and mixed the puffied fusion protein with Freund's adjuvant to iimnune rabbits in order to obtain specific rabbit anti-human LOH12CR1 antiserum, and identify the antisenaal with western blot. Results Suecessfitlly obtain the antiserum that can be, used to test the endogenous expression of LOH 12CR1. Conclusion The preparation of rabbit a...