期刊论文

中文

肿瘤药学

2095-1264

2023

13

06

719-728

10.3969/j.issn.2095-1264.2023.06.10

20200984:湖南省卫生健康委员会重点资助课题 2022007:湖南省中医药管理局重点项目

本校为第一机构

目的 基于网络药理学探究胃复方治疗胃癌的作用机制.方法 应用中药系统药理学数据库及分析平台、DrugBank、GeneCards等数据库检索胃复方所含12味中药的有效化学成分及治疗胃癌的潜在靶点,将潜在靶点导入DAVID数据库进行基因本体论(GO)及京都基因与基因组百科全书(KEGG)分析,筛选出潜在的生物过程和涉及的信号通路.采用CCK-8及Western blotting对KEGG分析富集程度最大的信号通路进行验证.结果 胃复方治疗胃癌可能涉及以槲皮素、木樨草素为代表的88种有效活性成分,可能作用于以TP53、STAT3、Akt为代表的194个靶点.GO分析涉及148个生物过程,KEGG分析筛选出PI3K/Akt、TNF、HIF-1等信号通路.体外实验...

Objective To explore the mechanism of Weifufang(an experienced effective prescription for the treatment of gastric cancer developed by Hunan Cancer Hospital)in treating gastric cancer on the base of network pharmacology.Meth-ods The effective active ingredients and potential targets for treating gastric cancer of 12 traditional Chinese medicines con-tained in Weifufang were searched by using the Chinese Medicine System Pharmacology Database and Analysis Platform,DrugBank,GeneCards and other databases.The potential targets were imported into DAVID database for gene ontology(GO)analysis and Kyot...