期刊论文

Effects of overtaking sour-flavored drug Cornus Officinalis on D cells of gastric mucosa,ET-1 and NO in rats

中文

湖南中医药大学学报

1674-070X

2010

30

7

19-21

10.3969/j.issn.1674-070X.2010.07.006.019.03

:中医临床基础重点学科建设资助项目

本校为第一机构

目的 观察过食酸味药山茱萸对大鼠胃窦黏膜D细胞和内皮素(ET-1)、一氧化氮(NO)的影响.方法 60只大鼠随机分为空白对照组、山茱萸临床等效剂量组、山茱萸低、中、高剂量组,分别给予蒸馏水和山茱萸水煎液灌胃.连续给药9周后,进行胃组织病理形态学检查,免疫组织化学法检测胃窦D细胞数,放射免疫法测血浆ET-1含量,硝酸还原法测血清NO含量.结果 低、中、高剂量组逐渐出现大鼠活动减少、倦卧嗜睡、食量减少、便溏、体质量减轻等中医脾虚症状.与空白对照组比较:(1)低、中、高剂量组的血浆ET-1含量、D细胞数及强阳性细胞率均明显升高,胃黏膜白细胞浸润程度明显加重,差异均具有统计学意义(P＜0.05,P＜0.01...

Objective To explore the effects of overtaking the sour-flavored Chinese drug cornus officinalis on D Cells of gastric mucosa,ET-1 and NO in rats.Methods 60 SD rats were divided randomly into control group,clinical equivalent dose group of cornus officinalis,and low,middle and high dose groups of cornus officinalis,and all of them were given water or decocted cornus officinalis liquid respectively 9 weeks.The histological changes were measured,the D cells were detected with immunicytochemistry,the contents of plasma ET -1 with radioimmunoassay ...