期刊论文

The determination of puerarin in Wuweianshen granules by reversed phase high performance liquid chromatography

中文

中国现代应用药学

1007-7693

2004

S1

63-65

10.13748/j.cnki.issn1007-7693.2004.s1.092

本校为其他机构

目的建立五味安神颗粒中葛根素的含量测定方法。方法 RP-HPLC色谱法,ODS C18柱,4.6 mm×150 mm（5μm）;流动相:甲醇-水（35:65）,内含0.01%醋酸;检测波长:252 nm;流速:1.2 mL.min-1。结果色谱分离良好,在0.020.64μg范围内成线性关系,Y=39443X+2477.6,r=0.9999,平均回收率为97.3%,RSD为1.58%。结论本方法简单、快速、准确,适合该制剂快速定量分析。

OBJECTIVE To establish a method for determination of puerarin in Wuweianshen granules containing puerariae radix.METHOD RP-HPLC,ODS column,methanol:H20(35:65) containing0.01%acetic acid as mobile phase at a flow-rat of 1.2 mL ? min ~,and detected at 252 nm.RESULTS Puerarin was eluted within 5 min without interference with co-existing components,the detection limits was 0.01 μg- 0.32μg(r = 0.9999),the average recovery was 97.3%with RSD = 1.58%.CONCLUSION This method is simple and accurat...