期刊论文

中文

中国医院药学杂志

1001-5213

2015

35

4

283-288

10.13286/j.cnki.chinhosppharmacyj.2015.04.02

81202985:国家自然科学基金 81473411:国家自然科学基金

本校为其他机构

目的:建立测定小鼠肝脏中16种胆汁酸浓度的LC-MS/MS法,并将测定结果应用于比较Nrf2野生型和基因敲除型小鼠胆汁酸代谢谱。方法:小鼠肝脏样品加入70%乙腈匀浆,用甲醇沉淀匀浆液中的蛋白并高速离心,取上清液置进样瓶, 用Xtimate~(TM) C_(18)柱分离,以甲醇-醋酸铵及甲酸水溶液为流动相进行梯度洗脱,流速0.25 ml·min~(-1),进样5 μl分析。结果: 16种胆汁酸在0.013~8.000 μmol·L~(-1)范围内,线性关系良好,定量下限浓度为0.013 μmol·L~(-1),肝脏匀浆液加样回收率在94.9%~112.8%之间,日内、日间RSD均小于10.8%。结论:该方法准确可靠,灵敏度高,处理简便,适用于肝脏中胆汁酸浓度的测定,为生物样品中...

OBJECTIVE To determine concentrations of 16 bile acids in mice liver by LC-MS/MS.METHODS After added70% acetonitrile into mice liver,protein precipitation with methanol was used for sample preparation.Liquid chromatographic separation was performed on a XtimateTMC18 column with gradient eluent consisted of methanol-ammonium acetate and formic acid buffer,at a flow rate of 0.25 ml·min^-1 and injection volume of 5μl.RESULTS The linear calibration curves were obtained in the concentration range of 0.013-8.000μmol·L^-1.The lower limit of quanti...