期刊论文

中文

中国现代应用药学

1007-7693

2017

34

1

25-29

10.13748/j.cnki.issn1007-7693.2017.01.007

湖南省教育厅科学研究项目（15A140） 2016年湖南中医药大学研究生科研创新课题（2016CX16）

本校为第一机构

目的观察六味地黄汤及其3个“一补一泻”药对对肾阴虚模型小鼠下丘脑-垂体-性腺(HPG)轴功能及性腺组织形态的影响。方法将60只小鼠随机分为空白对照组、模型组、六味地黄汤组、山茱萸-牡丹皮组、熟地黄-泽泻组、山药-茯苓组共6个试验组;连续9 d分别灌胃给予以上对应药物;第5~9天,除空白对照组灌胃蒸馏水外,其他各组按50 mg·kg~(-1)体质量每天灌胃氢化可的松注射液建立模型,用ELISA法检测血浆环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量,用放射免疫分析法检测促卵泡素(FSH)、雌二醇(E2)及睾酮(T)含量,并用HE染色法观察性腺(睾丸、子宫)病理切片。结果与空白对照组比较,肾阴虚模型小鼠血浆cAMP含量升...

OBJECTIVE To observe the effect of Liuwei Dihuang Decoction and three "reinforeing and reducing"herb couples on hypothalamus-pituitary-gonad (HPG) axis and morphologic changes of the gonad in kidney Yin deficiency mice. METHODS The mice were randomly divided into six experimental groups: blank group, model group, Liuwei Dihuang decoction group, Corni Fructus-Moutan Cortex couple group, Rehmanniae Radix Praeparata couple group and Dioscoreae Rhizoma-Poria couple group. The rats were gavaged with oral administration of corresponding medicines for...