期刊论文

Quantitative Determination of Three Phenolic Acids and Six Flavonoids of Phragmites Communis (Cav.) Trin. ex Steud from Yuanjiang by HPLC

中文

中国现代应用药学

1007-7693

2018

35

9

1317-1321

10.13748/j.cnki.issn1007-7693.2018.09.010

湖南省芦笋研究开发中心芦笋产业联合研究开发基金

本校为第一机构

目的建立同时测定沅江产芦笋中原儿茶酸、绿原酸、香草酸、柚皮苷、芦丁、槲皮素、木犀草素、山奈酚、芹菜素含量的HPLC。方法采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5 μm),以甲醇-0.1 %甲酸水溶液为流动相,梯度洗脱,双波长检测(酚酸:283,327 nm;黄酮:283,335 nm),流速1 mL·min~(-1),柱温35 ℃,进样量为10 μL。结果3种酚酸及6种黄酮分别在25 min和50 min内分离,线性范围为14.8~500 μg·mL~(-1)(r=0.999 0~0.999 7),检测限0.006 3~ 0.053 9 μg·mL~(-1),定量限0.021 1~0.179 7 μg·mL~(-1),加样回收率为98.73%~102.44%,RSD为1.27%~2.20%(n=6)。结论该方法准确灵敏、重复性好,可用于检...

OBJECTIVE To establish quantitative determination method of the protocatechuic acid, chlorogenic acid, vanillic acid, naringin, rutin, quercetin, luteolin, kaempferol and apigenin in Phragmites communis (Cav.) Trin. ex Steud from Yuanjiang by HPLC. METHODS The chromatographic separation was performed on a Diamonsil C_(18) column (250 mm× 4.6 mm, 5 μm) kept at 35 ℃, using methanol-0.1% formic acid aqueous as the mobile phase at a flow rate of 1 mL·min~(-1) through gradient elution, and the detection wavelength was set at dual wavelength(phen...