期刊论文

Simultaneous determination of 15 bile acids in ICR mice liver by LC-MS/MS and its application in triptolide induced hepatotoxicity research

中文

中国医院药学杂志

1001-5213

2018

38

11

1138-1143

10.13286/j.cnki.chinhosppharmacyj.2018.11.02

81202985:国家自然科学基金 81573686:国家自然科学基金 81703818:国家自然科学基金

本校为第一机构

目的:建立同时测定ICR小鼠肝脏中15种胆汁酸的LC-MS/MS法,将其应用到雷公藤甲素肝毒性研究中.方法:小鼠经腹腔注射1 mg·kg-1雷公藤甲素后,进行血清生化、肝病理切片检查.将肝脏用0.1％甲酸-75％乙腈混合液进行匀浆并经样品处理,采用Ultimate(R) AQ-C18色谱柱,流速0.3 mL·min-1,进行15种胆汁酸色谱分离.结果:LC-MS/MS法测定的15种胆汁酸在2.8～8 000.0 nmol· L-1浓度范围内具有较好的线性关系,加样回收率在85.8％～114.0％之间,日内、日间精密度均在0.7％～10.8％范围内;腹腔注射1mg·kg-1雷公藤甲素后小鼠出现了肝毒性,雷公藤甲素组的胆汁酸浓度有明显上升.结论:该方法准确、稳定性好、灵敏度...

OBJECTIVE To develop a liquid chromatography-tandem mass spectrometry（LC-MS/MS）method for simultaneous determination of 15 bile acids in the liver of ICR mice,and its application to a triptolide induced hepatotoxicity research.METHODS The serum biochemical indices and liver histopathology were detected after intraperitoneal injecting of triptolide at the dose of 1.0 mg·kg^-1.The liver was homogenized with the 0.1% formic acid-75% acetonitrile mixture.Liquid chromatographic separation of 15 bile acids was performed on an UltimateAQ-C18 colu...