期刊论文

Pharmacokinetics of myricetin, dihydromyricetin from ampelopsis grossedentata and its acetylated derivatives in SD rats

中文

中国医院药学杂志

1001-5213

2019

39

22

2266-2272

10.13286/j.cnki.chinhosppharmacyj.2019.22.04

国家自然科学基金(编号:81573686,81703818) 湖南省自然科学基金(编号:2019JJ50680) 长沙市科技计划项目经费资助(编号:kq1801123)。

本校为第一机构

目的:建立LC-MS/MS法测定SD大鼠血浆中杨梅素(myricetin,MY)和二氢杨梅素(dihydromyricetin,DMY)的浓度,将其运用到药动学研究,并评价DMY乙酰化衍生物是否改善生物利用度。方法:SD大鼠随机分为4组,分别灌胃DMY (100 mg·kg^-1,相当于9 375.0μmo·L^-1)的0.5%羧甲基纤维素钠生理盐水溶液,以及等摩尔剂量的DMY-1、DMY-2、MY。于给药前0.0 h和给药后0.08,0.17,0.25,0.33,0.50,0.75,1.0,2.0,4.0,6.0,8.0,12.0 h尾静脉断尾采血200μL,血浆样品经0.1%甲酸-乙腈沉淀蛋白,内标法定量,DAS3.0和SPSS统计分析得到药动学参数和曲线。结果:低、中、高的质控样品日内、日间精密度RSD均小于10.2%;DMY和MY的基质...

OBJECTIVE To establish an LC-MS/MS method for the determination of myricetin(MY) and dihydromyricetin(DMY) in SD rat plasma and to evaluate whether DMY acetylated derivatives improve the bioavailability.METHODS SD rats were randomly divided into four groups and received intragastric administration of DMY(100 mg·kg^-1, equivalent to 9 375.0 μmo·L^-1) in normal saline containing 0.5% sodium carboxymethyl cellulose, and equimolar doses of DMY-1, DMY-2, MY. 200 μL blood samples were collected from the tail vein at 0.0 h before administration an...