期刊论文

Effect of Tianma Xiongling Zhixuan Tablet on Blood Pressure and Vascular Remodeling in Spontaneously Hypertensive Rats

中文

中国现代应用药学

1007-7693

2020

37

4

414-419

10.13748/j.cnki.issn1007-7693.2020.04.006

国家自然科学基金项目(81473616) “重大新药创制”国家科技重大专项(2019ZX09301103) 中国博士后科学基金项目(2018M632966) 湖南省卫生健康委科研计划项目(C2019090) 长沙市杰出创新青年培养计划项目(kq1802008) 湖南省高校“双一流”建设项目(湘教通[2018]469号)。

本校为第一机构

目的 探讨天麻芎苓止眩片(Tianma Xiongling Zhixuan tablet,TXZT)对自发性高血压大鼠(spontaneously hypertensive rats,SHR)的降压作用和改善血管重塑作用.方法 采用SHR大鼠和正常WKY大鼠进行实验,连续给药4周,比较每周各组大鼠血压值;ELISA法检测血清中eNOS、ET-1、TXA2、AngⅡ的含量变化;HE染色法观察大鼠主动脉的病理变化;免疫组化和Western blotting测定主动脉MMP-2、TIMP-2蛋白表达;RT-PCR测定主动脉MMP-2、TIMP-2 mRNA表达.结果 与模型组比较,给药第1～4周,TXZT具有显著的降血压药理作用(P＜0.01或P＜0.05).与模型组相比,TXZT能够有效降低SHR大鼠血清ET-1、TXA2、AngⅡ的水平,升高eNOS的...

OBJECTIVE To explore the anti hypertensive pharmacological mechanism and vascular remodeling mechanism of Tianma Xiongling Zhixuan tablet(TXZT) in spontaneously hypertensive rats(SHR). METHODS SHR and WKY rats were used. The rats were continuously intragastrically administered for 4 weeks, observed and compared the blood pressure values of each group in each week. The levels of eNOS, ET-1, TXA2 and AngⅡ in serum were detected by ELISA. The pathological changes of rat thoracic aorta were observed by HE staining. The expression of MMP-2 and TIMP...