期刊论文

Study on the Mechanism of Epimedii Folium in Treating Alzheimer's Disease Based on Network Pharmacology

中文

中国现代应用药学

1007-7693

2021

38

16

1945-1951

10.13748/j.cnki.issn1007-7693.2021.16.004

国家自然科学基金项目（81874462）；

本校为第一机构

目的基于网络药理学,探讨淫羊藿治疗阿尔茨海默病（Alzheimer’s disease,AD）的作用靶点及通路,明确其作用机制。方法借助TCMSP数据库及Uniprot数据库筛选出淫羊藿有效成分及靶点基因。通过Drugbank、DisGeNET和TTD数据库筛选出AD的靶点基因;成分靶点与疾病靶点映射后使用Cytoscape 3.7.1软件构建药物有效成分-靶点蛋白相互作用网络,使用STRING数据库绘制靶点蛋白-靶点蛋白相互作用网络;对靶点蛋白利用Metascape数据库进行GO分析和KEGG分析,最后采用MTT试验和荧光实时定量PCR对网络药理学主要分析结果进行验证。结果筛选得到23个淫羊藿活性成分,共预测获得239个淫羊藿药效靶点和2 156个AD的治疗...

OBJECTIVE To explore the target and pathway of Epimedii Folium in the treatment of Alzheimer's disease(AD)based on network pharmacology,and to clarify its mechanism.METHODS The effective components and target genes of Epimedii Folium were screened by TCMSP database and Uniprot database.The target genes of AD were screened by Drugbank,Dis Ge NET and TTD databases.After mapping the component target with the disease target point,Cytoscape 3.7.1 software was used to construct the drug active component-target protein interaction network,at the same ...