期刊论文

Zhou, X.

中文

中国现代应用药学

1007-7693

2022

39

2

154-160

10.13748/j.cnki.issn1007-7693.2022.02.003

国家自然科学基金项目（81874462）； 重庆市中医药重点学科（中医基础理论）建设项目（渝中医[2021]16号）；

本校为通讯机构

目的基于网络药理学探讨六味地黄丸治疗阿尔茨海默病（Alzheimer’s disease,AD）的作用机制。方法从TCMSP数据库筛选六味地黄丸治疗AD的活性成分,并构建药物-靶标网络、蛋白与蛋白相互作用（protein-protein interaction,PPI）网络,运用生物信息学分析方法进行GO分析、KEGG通路富集分析和PPI网络分析。采用荧光实时定量PCR对网络药理学主要分析结果进行验证。结果分析结果表明,六味地黄丸主要关联AD的β淀粉样蛋白聚集、Tau蛋白的磷酸化、细胞凋亡、氧化应激反应、炎症反应、自噬、胰岛素代谢等;体外实验提示,六味地黄丸参与调控APP介导的β淀粉样蛋白聚集,GSK-3β介导的Tau蛋白磷酸化,GNB1介导...

OBJECTIVE To explore the mechanism of Liuwei Dihuang pills in the treatment of Alzheimer's disease(AD) based on network pharmacology. METHODS The active ingredients of Liuwei Dihuang pills in the treatment of AD were screened from the TCMSP database, and the drugs-targets network and protein-protein interaction(PPI) network were constructed. GO analysis, KEGG pathway enrichment analysis and PPI network analysis were performed by bioinformatics analysis methods. The main results of network pharmacology were verified by real-time quantitative PCR...