期刊论文

Implications of Thl7 cells and its regulatory cytokines in patients with middle to late staged nasopharyngeal carcinoma

中文

中国中西医结合耳鼻咽喉科杂志

1007-4856

2013

4

244-248

10.3969/j.issn.1007-4856.2013.04.002

湖南省中医药科研基金重点项目（2010007） 湖南省重点学科基金中医五官科学专项.

本校为第一机构

目的探讨Th17细胞功能活性及其相关调节性细胞因子在中晚期鼻咽癌患者免疫病理中的意义。方法 28例中晚期鼻咽癌患者,应用流式细胞术检测外周血Th17细胞数量比例,RT-PCR法检测ROR-γt mRNA转录水平,ELISA法检测血清白介素10（IL-10）和17（IL-17）及γ干扰素（IFN-γ）含量水平,并以15例健康人作为对照,分析其组间差异及其意义。结果中晚期鼻咽癌患者外周血单个核细胞中Th17细胞占CD4+T细胞的数量比例和ROR-γt mRNA的相对表达量均低于正常对照组（P

Objective To investigate the implications of Th17 cells and its regulatory cytokines in patients with middle to late staged nasopharyngeal carcinoma （NPC） based on a clinical trial. Methods The ratio of Th17 cells in the peripheral blood mononuclear cells （PMBC） was detected among 28 patients with middle to late stage of NPC by the procedures of flow cytometry, and ROR-γt mRNA in PMBC was determined by RT-PCR technique. Furthermore, serum levels of IL-10, IL-7 and IFN-γ were assayed by the use of ELISA Meanwhile, 15 healthy persons were t...