期刊论文

Study on effects and underlying mechanisms of combined antelope horn with uncaria in rats in development period with febrile seizures

中文

中国免疫学杂志

1000-484X

2018

34

8

1183-1186,1194

10.3969/j.issn.1000-484X.2018.08.012

湖南省科技计划项目（2015JC3076）资助；

本校为第一机构

目的:探讨羚羊角与钩藤联合用药对热性惊厥所致发育期大鼠脑损伤的保护作用及其作用机制.方法:采用热水浴建立热性惊厥大鼠模型,实验分为5组,空白对照组、模型组、羚羊角组、钩藤组、羚羊角与钩藤联合用药组(简称联用组),每组10只,共50只.通过HE染色法观察组织病理情况;ELISA法检测各组大鼠脑组织TNF-α、IL-1β、Glu及Asp含量;免疫组化法检测大鼠海马区TNF-α、IL-1β 表达情况.结果:联用组、羚羊角组、钩藤组脑组织Asp、Glu、TNF-α 及IL-1β 及海马区TNF-α、IL-1β 含量显著低于模型组(P

Objective： To investigate effects and underlying mechanisms of combined antelope horn and uncaria in rats in development period with febrile seizures. Methods： Model of febrile seizures were established by hot bath,and randomly divided into 5 groups： the control group,the model group,the antelope horn group,the uncaria group,and the combined antelope horn and uncaria group,each for 10 rats,and total 50 rats. The levels of brain tissues of Glu,Asp,TNF-α and IL-1β were detected by ELISA,and the expressions of hippocampus of TNF-α and IL-1β...