期刊论文

Therapeutic effect of Kechuanning on RSV-induced asthmatic rats and its effect on IL-33 expression

中文

中国免疫学杂志

1000-484X

2020

36

17

2120-2125

10.3969/j.issn.1000-484X.2020.17.015

No.81674025:国家自然科学基金 No.81774368:国家自然科学基金 2015JJ2016:湖南省自然科学基金 2018-16:湖南中医药大学大学生研究性学习和创新性实验课题

本校为第一机构

目的:探讨咳喘宁对病毒诱发哮喘大鼠的治疗作用及对IL-33表达的影响。方法:以呼吸道合胞病毒(RSV)滴鼻诱导+卵清白蛋白致敏的方法制作哮喘模型,实验动物随机分为正常组、模型组、咳喘宁高剂量组、咳喘宁中剂量组和咳喘宁低剂量组,各组分别给予生理盐水和相应药物处理。末次给药后进行大鼠气道高反应性测定;HE染色和Masson染色分别观察肺组织病理学改变和气道下胶原沉积情况;ELISA法检测血清中IL-33水平,qPCR法检测肺组织IL-33 mRNA表达。结果:与正常组相比,模型组大鼠哮喘症状严重,气道反应性升高,肺组织结构破坏,气道下胶原沉积增多(P

Objective:To investigate therapeutic effect of Kechuanning on virus-induced asthmatic rats and its effect on IL-33 expression.Methods:Asthma model was established by method of respiratory syncytial virus(RSV)nasal induction and ovalbumin sensitization,experimental animals were randomly divided into normal group,model group,Kechuanning high dose group,Kechuanning middle dose group and Kechuanning low-dose group,each group was given control saline and corresponding drugs.Airway hyperrespons-iveness was measured after the last administration;pathological changes of lung tissue and collagen deposi...