期刊论文

Effect of Yixintai Pill on cardiomyocyte apoptosis in rats with cardiac failure caused by adriamycin and its mechanism

中文

中国免疫学杂志

1000-484X

2020

36

21

2592-2596

10.3969/j.issn.1000-484X.2020.21.007

湖南省中医药管理局课题（201833）；

本校为第一机构

目的:探讨益心泰丸保护阿霉素(ADR)致心力衰竭(HF)大鼠心肌细胞凋亡的影响及作用机制。方法:将60只健康雄性Wistar大鼠随机分为4组,空白对照组、模型组、开博通组、益心泰丸组,腹腔一次性注射阿霉素(2.5 mg /kg)构建HF大鼠模型,对照组灌胃给予等体积生理盐水,益心泰丸组和开博通组分别灌胃给予相应药物5 mg /kg,用药4周。停药后空腹采血检测PRA、AngⅡ、ALD的含量,B超检测心脏结构,计算心体比,HE染色观察大鼠心肌组织病变,TUNEL法观察心肌细胞凋亡,ELISA法检测血清TNF-α和IL-6的表达,Western blot检测心肌组织中Akt、p-Akt、GSK-3β、P-GSK-3β蛋白的表达。结果:模型组PRA、AngⅡ、ALD、LVPWs、...

Objective: To investigate the effect and mechanism of Yixintai Pill on cardiomyocyte apoptosis induced by adriamycin(ADR) in rats with heart failure(HF).Methods: 60 healthy male Wistar rats were randomly divided into four groups: blank control group,model group, captopril group and Yixintai Pill group.HF rat models were established by intraperitoneal injection of adriamycin(2.5 mg /kg).The control group was given the same volume of normal saline, and Yixintai Pill group and captopril group were given the corresponding drugs 5 mg /kg for 4 weeks...