期刊论文

Effect of JiangzhiLigan Decoction on non-canonical pyroptosis pathway in rats with high-fat diet-induced non-alcoholic fatty liver disease

中文

中国免疫学杂志

1000-484X

2020

36

3

267-271

10.3969/j.issn.1000-484X.2020.03.003

81503385:国家自然科学基金 C2017028:湖南省卫计委科研计划项目 18B244:湖南省教育厅科学研究项目 18B252:湖南省教育厅科学研究项目 No.06:湖南中医药大学十三五一级学科基础医学建设项目

本校为第一机构

目的:观察降脂理肝汤对非酒精性脂肪肝病(NAFLD)大鼠非经典的细胞焦亡途径的影响,探讨降脂理肝汤干预非酒精性脂肪肝的机制。方法:利用高脂饮食喂养制备大鼠NAFLD模型,给予降脂理肝汤连续灌胃6周干预后,Western blot检测大鼠肝脏组织GSDMD和Caspase-11蛋白表达水平,试剂盒检测大鼠门静脉血清LPS以及血清IL-1β、IL-18、TNF-α 和IL-6水平。结果:与正常组比较,大鼠肝脏组织GSDMD、GSDMD的N端(GSDMD-N)以及Caspase-11蛋白水平明显升高,大鼠门静脉血清LPS以及血清IL-1β、IL-18、TNF-α 和IL-6水平显著升高;降脂理肝汤干预后,大鼠肝脏组织GSDMD、GSDMD-N以及Caspase-11蛋白水平明显降低,大鼠门静脉...

Objective:To explore the effect of JiangzhiLigan Decoction on the non-canonical pyroptosis pathway of rats with non-alcoholic fatty liver disease(NAFLD).Methods:NAFLD rat model was established by high-fat diet feeding.Rats were orally administered with of JiangzhiLigan Decoction for 6 weeks.Western blot was used to detect the expression levels of GSDMD,GSDMD-N terminal(GSDMD-N)and Caspase-11 protein in the liver tissue.The portal LPS levels and the serum levels of IL-1β,IL-18,TNF-α,and IL-6 were determined by test kits.Results:The levels of G...