期刊论文

Study on effect of Zuogui compound on intestinal inflammation and mucosal barrier in type 2 diabetic mice based on theory of "spleen-based virtual"

中文

中国免疫学杂志

1000-484X

2020

36

6

667-671,676

10.3969/j.issn.1000-484X.2020.06.006

2018YFC17044301:国家重点研发计划 2018YFC1704402:国家重点研发计划 81573956:国家自然科学基金 2018TP1021:湖南省重点实验室项目 2018JJ3394:湖南省自然科学基金 201931:湖南省中医药科研项目 16C1235:湖南省教育厅科学研究项目 18A220:湖南省教育厅科学研究项目 2017-1:湖南中医药大学校级科研基金项目 CX2018B483:湖南省研究生科研创新项目 CX2018B467:湖南省研究生科研创新项目 2017-1:湖南中医药大学大学生研究性学习和创新性实验计划

本校为第一机构

目的:探讨2型糖尿病(T2DM) “脾为本虚”的理论机制及左归复方对T2DM肠道炎症及黏膜屏障的影响及作用机制。方法:采用2型糖尿病MKR模型鼠,连续给予左归复方4周,ELISA法检测胰岛素、炎症相关因子及内毒素浓度, HE染色观察肠道病理形态,RT-qPCR和Western blot检测肠道TLR4 /NF-κB通路和紧密连接蛋白的基因和蛋白表达。结果:左归复方可明显降低MKR鼠血糖水平,改善胰岛素抵抗;左归复方可明显抑制炎症因子(IL-1β、IL-6、TNF-α)分泌,降低血液中内毒素浓度;左归复方还能上调肠道紧密连接蛋白Occludin和ZO-1的表达,下调TLR4 /NF-κB信号通路的mRNA和蛋白表达。结论:左归复方可通过减轻肠道慢性低度炎...

Objective: To investigate the theoretical mechanism of " spleen-based deficiency" in T2DM and the effect and mechanism of Zuogui compound on T2DM intestinal inflammation and mucosal barrier. Methods: MKR mice received gavage of Zuogui compound for 4 weeks. Insulin,inflammation-related factors and endotoxin concentrations were detected by ELISA. Pathological morphology of intestinal tissues was observed by HE staining. Expression of TLR4 /NF-κB pathway and tight junction protein genes and proteins were detected by RT-qPCR and Western blot. Resu...