期刊论文

Gufei Xiaoji Decoction inhibits lung cancer A549 cell migration and invasion by up-regulating exosomal microRNA-186-5p expression

中文

中国免疫学杂志

1000-484X

2021

37

23

2856-2860

10.3969/j.issn.1000-484X.2021.23.008

湖南省中医药研究院科研课题（201704）资助；

本校为第一机构

目的:研究外泌体途径在固肺消积饮抑制肺癌A549细胞迁移和侵袭中的作用,及microRNA-186-5p(miR-186-5p)在其中的作用机制。方法:制备固肺消积饮含药血清和无药血清,分别诱导A549细胞、转染miR-186-5p NC的A549细胞、转染miR-186-5p inhibitor的A549细胞,获得4种外泌体,即NC-Exo、XJD-Exo、XJD-miR-186-5p NC-Exo、XJD-miR-186-5p inhibitor-Exo。透射电镜观察外泌体形态;Western blot检测CD63、TSG101和Calnexin表达;荧光标记法检测A549细胞摄取外泌体情况;RT-qPCR检测外泌体中miR-186-5p表达。实验分为:A组(A549细胞+PBS)、B组(A549细胞+NC-Exo)、C组(A549细胞+XJD-Exo)、D组(A549细胞+XJD-miR...

Objective:To investigate role of exosome pathway in inhibiting migration and invasion of lung cancer A549 cells by Gufei Xiaoji Decoction and mechanism of microRNA-186-5p(miR-186-5p).Methods:Gufei Xiaoji Decoction-containing serum and drug-free serum were prepared and used to stimulate A549 cells,A549 cells transfected with miR-186-5p NC,and A549 cells transfected with miR-186-5p inhibitor.Four exosomes,namely NC-Exo,XJD-Exo,XJD-miR-186-5p NC-Exo,XJD-miR-186-5p inhibitor-Exo,were obtained.Morphology of exosomes was observed by transmission electron microscope.Expressions of CD63,TSG101 and Cal...