期刊论文

Effects of Dahuang Lingxian prescription on Wnt5a-Ca~(2+)-PKC signal transduction pathway and inflammatory response in rats with intrahepatic bile duct injury induced by LPS

中文

中国免疫学杂志

1000-484X

2022

38

1

39-44

10.3969/j.issn.1000-484X.2022.01.007

广西自然科学基金项目（2018JJA140857）； 广西壮族自治区科学技术厅资助项目（2019JJB140120）；

本校为第一机构

目的:探讨大黄灵仙方对LPS诱导的肝内胆管组织损伤大鼠Wnt5a-钙离子(Ca~(2+))-活化蛋白激酶C(PKC)信号转导通路及炎症反应的影响。方法:SD大鼠随机分为正常对照组、模型组、大黄灵仙方组(320 mg/kg)、炎症信号阻断剂组(PDTC+SB203580,120 mg/kg+10 mg/kg)、大黄灵仙方+信号阻断剂组(320 mg/kg+120 mg/kg+10 mg/kg),每组12只。采用胆总管注射内毒素LPS构建肝内胆管组织损伤模型,分别于造模前和造模后连续给药3 d,2次/d。末次给药12 h后,ELISA检测血清总胆红素(TBIL)、总胆汁酸(TBA)及肿瘤坏死因子(TNF-α)、IL-6水平;截取胆管周围组织,HE染色观察组织病理变化;荧光免疫法检测胆管组织细胞内Ca~(2...

Objective:To investigate effects of Dahuang Lingxian prescription on Wnt5a-calciumion(Ca~(2+))-activated protein kinase C(PKC)signal transduction pathway and inflammatory response in rats with intrahepatic bile duct injury induced by LPS.Methods:SD rats were randomly divided into normal control group,model group and Dahuang Lingxian prescription group(320 mg/kg),inflammation signal blocker group(PDTC+SB203580,120 mg/kg+10 mg/kg),Dahuang Lingxian prescription+signal blocker group(320 mg/kg+120 mg/kg+10 mg/kg),with 12 rats in each group.Intrahepa...