期刊论文

中文

中国药师

1008-049X

2013

16

10

1459-1460

10.3969/j.issn.1008-049X.2013.10.006

基金项目：国家药典委员会国家药品标准提高行动计划项目（编号：218）

本校为第一机构

目的:建立高效液相色谱法测定四物颗粒中阿魏酸含量的方法.方法:色谱柱为Agilent C18(TC) (250 mm×4.6 mm,5 μm),流动相为甲醇-0.1%磷酸 (30:70),流速为0.8 ml·min-1,检测波长为321 nm,进样量10 μl,柱温35 ℃.结果:阿魏酸对照品进样量在4.99～208.00 ng范围内线性关系良好(r=0.999 8),平均加样回收率为101.79%,RSD为1.33%(n=6).结论:该方法简便、准确、可靠,可用于四物颗粒中阿魏酸的含量测定.

Objective ： To establish a method for the determination of ferulic acid in Siwu granules by HPLC. Method ： A column ofAgilent C18 （TC） （ 250 mm ×4.6 mm,5 μm）with the temperature of 35℃ was used to separate the target component, the mobile phase was composed of methanol-0, 1% phosphate solution （30： 70） , the flow rate was 0.8 ml ·min^-1 and the detection wavelength was at 321 nm. Result： The linear range of ferulic acid was 4.99-208.00 ng（ r = 0.999 8 ）, and the＇average recovery was 101.79% with RSD of 1.33% （n = 6）. Conclusi...