期刊论文

Study on Preparation Process of Dendrobium Candidum Eye Drops

中文

中国药师

1008-049X

2016

19

1

73-77

10.3969/j.issn.1008-049X.2016.01.021

湖南省科技计划重大专项（编号:2010FJ1010-3）；

本校为第一机构

目的:优选铁皮石斛多糖提取、纯化及滴眼剂配制的工艺参数,为工业化生产提供依据。方法:采用硫酸-苯酚比色法测定铁皮石斛样品中的多糖含量;采用单因素和正交试验优选水提、醇沉的工艺参数;采用等级一致性检验法优选滴眼液的配制参数。结果:最佳水提工艺为:铁皮石斛鲜药材经冷冻干燥（-55℃、-0.0120Mpa）后,粉碎、过40目筛,过筛后粉末加30倍量水浸泡120h,提取3次,提取时间分别为3,2.5,220h,第2、3次提取加水量分别为25倍和20倍,提取温度为85℃。最佳醇沉工艺为:提取液浓缩至固液比为1∶3时,加乙醇使含醇量达85%,静置2420h。滴眼液配制参数为:采用3%聚氧乙烯氢化蓖麻油作为稳定剂,0.25%苯甲酸钠...

Objective： To research the best extraction and purification technology for dendrobium candidum polysaccharide and the optimal preparation parameters for its eye drops to provide basis for the industrial production. Methods： By a sulfuric acid-phenol eolorimetrie method, the content of dendrobium eandidum polysaccharide was determined. The process parameters of water extraction and alcohol precipitation were optimized by the single factor test and orthogonal test. The formula parameters of eye drops were optimized by a level consistency check ...