期刊论文

Determination of Dracohodin in Qilisan Gel by HPLC

中文

中国药师

1008-049X

2016

19

3

589-591

10.3969/j.issn.1008-049X.2016.03.053

湖南省自然科学基金资助项目（编号:13JJ5033）；

本校为第一机构

目的：建立七厘散凝胶剂工艺研究中质量控制指标血竭素含量的测定方法,并进行方法学考察,为七厘散凝胶剂的工艺研究提供质量控制依据。方法：采用高效液相色谱法（HPLC）,色谱柱为DiamonsilRC_（18）（250 mm×4.6 mm,5μm）;检测波长为440 nm;柱温为30℃;采用乙腈-0.05 mol·L（-1）磷酸二氢钠溶液（45∶55）作为流动相;流速为l.0 ml·min（-1）。结果：血竭素含量在1.63264.250μg·ml（-1）范围内具有良好线性关系（r=0.999 6）;平均加样回收率为99.02%,RSD=0.77%（n=6）。理论塔板数按血竭素高氯酸盐色谱峰计算为7 100,且阴性无干扰。结论：本方法客观、准确、灵敏、可信度高,且操作简便快速...

Objective: To establish a method for determining the content of dracorhodin as an indicator in the quality control with methodological studies to provide basis for the preparation process study of Qilisan gel. Methods: An HPLC method was used with a DiamonsilR C18 (250 mm ×4. 6 mm, 5 μm) column, the detection wavelength was set at 440 nm and the column temperature was 30 ℃. Acetonitrile-0. 05 mol·L-1 sodium dihydrogen phosphate solution (45 ∶ 55) was used as the mobile phase, and the flow rate was l. 0 ml·min-1 . Results: The content of d...