期刊论文

HPLC Fingerprint of the. Ingredients in Simiao Junyi Ointment

中文

中国药师

1008-049X

2017

20

1

38-42

10.3969/j.issn.1008-049X.2017.01.010

湖南省科技厅计划项目（编号：20095K3125） 长沙市科技局计划课题项目（编号：K1508030-31、K1003068-31）

本校为第一机构

目的：：建立四妙君逸软膏HPLC指纹图谱分析方法,为制定其质量控制标准提供依据。方法：建立了分析四妙君逸软膏中主要成分的HPLC指纹图谱分离条件,即：色谱柱Ultimate C18-ODS柱(250 mm ×4.6 mm,5μm)；流动相为乙腈-0.1%磷酸水；流速：1.0 ml·min-1；检测波长：280 nm ；柱温：35℃,并且对色谱图中的共有峰进行了归属分析。结果：在筛选的最佳分离条件下进行梯度洗脱,四妙君逸软膏中各组分分离效果较好,可得到最优的指纹图谱；经方法学考察表明,所建立的HPLC指纹图谱分析方法精密度、稳定性、重复性均良好,指纹图谱清晰、可辨、易分析；初步确定四妙君逸软膏与四味药材指纹图谱共有峰之间的...

Objective ： To establish an analytical method for HPLC fingerprint chromatography of Simiao Junyi ointment to provide basis for the quality control standard. Methods： The separation conditions were established to obtain the HPLC fingerprint chromatog- raphy of the main ingredients in Simiao Junyi ointment. The conditions were as follows ： the chromatographic column was Ultimate C1s- ODS（250 mm ×4.6 mm,5 μm）, the mobile phase was acetonitrile-0.1% phosphate solution, the flow rate was 1.0 ml min-1 , the detection wavelength was 280 nm, an...