期刊论文

Simultaneous Determination of Eight Flavonoids in the Leaves of Acanthopanax Evodiaefolius Franch by HPLC

中文

中国药师

1008-049X

2019

22

9

1725-1728

10.3969/j.issn.1008-049X.2019.09.040

湖南省自然科学基金项目(编号:2019JJ40223) 湖南省自然科学基金青年基金项目(编号:2019JJ50448) 湖南中医药大学生物工程重点学科资助(校行科字[2018]3号) 湖南省研究生创新科研项目(编号:CX2017B444).

本校为第一机构

目的:采用HPLC法同时检测吴茱萸五加叶中8种黄酮类成分的含量,以期为该植物的进一步研究提供参考.方法:采用Promosil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1％磷酸,梯度洗脱,流速1.0 ml·min-1,柱温30℃,检测波长283 nm.结果:在优化的色谱条件下,8种成分均有良好的分离度,以及良好的线性关系,方法精密度RSD＜2.0％,待测样品于室温下24h内能保持稳定,apig-enin-6,8-C-di-β-D-glucopyranoside、芦丁、金丝桃苷、柚皮苷、樱桃苷、芹菜素-7-O-新橙皮苷、大波斯菊苷、槲皮素的线性范围分别为7.32～ 146.40 μg·ml-1(r1 =0.999 7)、10.20～204.00 μg·ml-1(r2=0.999 2)、4.26～85.20 μg·m...

Objective:To determine the contents of 8 flavonoids in the leaves of Acanthopanax evodiaefolius by HPLC in order to provide reference for the further study.Methods:The analysis was performed on a Promosil C18 column(250 mm×4.6 mm,5μm)eluted with acetonitrile and 0.1%phosphoric acid with gradient elution.The flow rate was 1.0 ml·min-1,the column temperature was30℃,and the detection wavelength was 360 nm.Results:The eight compounds were separated perfectly under the optimized conditions,and the regression equation showing linear relationship ...