期刊论文

Study on the Static and Dynamic Adsorption Properties of Total Flavonoids and Total Phenolic Acids in Asparagus from Gramineae by HPD100 and D101 Macroporous Adsorbent Resins

中文

中国药师

1008-049X

2020

23

5

839-844

10.3969/j.issn.1008-049X.2020.05.010

长沙市科技计划项目(编号:kq1701070) 湖南中医药大学与沅江市政府科研合作基金项目(编号:201606) 湖南中医药大学一层次学科中药学学科项目(校行科字[2018]3号)。

本校为第一机构

目的:将HPD100与D101大孔吸附树脂纯化禾本科芦笋总黄酮与总酚酸,优选总黄酮与总酚酸纯化工艺。方法:测定静态吸附率、解吸率、静态吸附曲线来评价HPD100大孔吸附树脂与D101大孔树脂对芦笋中总黄酮与总酚酸的静态吸附性能;然后对径高比、洗脱溶剂、水洗体积、洗脱剂量等考察获取动态纯化工艺。结果:总黄酮与总酚酸在HPD100大孔树脂上静态吸附量为分别为38.23 mg·g-1与22.40 mg·g-1,解吸率大小分别为68.95%与79.05%。动态优选的结果为浓度1∶0.25,上样量4 BV,径高比1∶4,静置4 h,9 BV水洗,50 ml 90%乙醇洗脱,总黄酮与总酚酸纯度分别达34.90%与20.07%。总黄酮与总酚酸在D101大孔树脂上静态吸附量...

Objective:To obtain the purification process of total flavonoids and phenolic acids in Asparagus from Gramineae by studying the purification of total flavonoids and phenolic acids by HPD100 and D101 macroporous resins.Methods:The static adsorption rate,desorption rate and static adsorption curve were determined to evaluate the static adsorption performances of HPD100 and D101macroporous resins for total flavonoids and total phenolic acid in Asparagus,and then the dynamic purification process was obtained by investigating the diameter-height rat...