期刊论文

中文

中国药师

1008-049X

2020

23

9

1835-1837

10.3969/j.issn.1008-049X.2020.09.039

国家自然科学基金青年基金项目(编号:81803729) 湖南省研究生创新课题(编号:CX2018B507) 长沙市科技局平台和人才计划项目(编号:kc1701009)。

本校为第一机构

目的:探讨不同炮制方法对青龙衣中胡桃醌含量的影响。方法:采用HPLC法测定青龙衣不同炮制品中胡桃醌的含量,色谱柱Agilent TC-C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(30∶70),流速:1 ml·min^-1,柱温:30℃,检测波长:250 nm,进样量:20μl。结果:胡桃醌线性范围为0.2~1.0 mg·ml^-1(r=0.9998),平均回收率为98.54%,RSD为1.97%(n=9),青龙衣不同炮制品的胡桃醌含量存在差异,生品胡桃醌含量最高,盐炙品、酒炙品、醋炙品、姜炙品次之,蜜炙品最低。结论:不同的炮制方法对青龙衣中的胡桃醌含量有影响,可为青龙衣炮制工艺的改进和新工艺的建立提供科学依据。

Objective:To explore the effect of different processing methods on the content of Juglone in green walnut husks.Methods:HPLC was adopted to detect the content of Juglone in different processed products of green walnut husks.The chromatographic column was Agilent TC-C18(150 mm×4.6 mm,5μm).The mobile phase was methanol-water(30∶70)at the flow rate of 1 ml·min^-1;the column temperature was 30℃;the detection wavelength was 250 nm;the injection volume was 20μl.Results:The linear range of juglone was 0.2-1.0 mg·ml^-1(r=0.9998),the average reco...