期刊论文

中文

中华中医药学刊

1673-7717

2010

28

4

802-804

10.13193/j.archtcm.2010.04.132.liujh.048

基金项目：湖南省科技厅资助项目（2007TP4021,湘财教指2009-42） 湖南省教育厅资助项目（08C637,09C724）

本校为第一机构

目的:研究壮骨镇痛胶囊对高转移人乳腺癌MDA-MB-231细胞迁移及其运动信号通路关键蛋白P-JNK和Rac-1表达水平的影响.方法:采用划痕法测定高(5.0%)、中(1.25%)、低(0.63%)不同浓度壮骨镇痛胶囊含药血浆对MDA-MB-231细胞迁移的影响,采用免疫细胞化学法检测不同浓度壮骨镇痛胶囊对MDA-MB-231细胞P-JNK和Rac-1蛋白表达水平的影响.结果:高、中浓度壮骨镇痛胶囊含药血浆显著抑制了乳腺癌MDA-MB-231细胞的迁移和P-JNK、Rac-1蛋白表达,低浓度血浆对细胞迁移和P-JNK、Rac-1蛋白表达无显著影响.结论:壮骨镇痛胶囊可通过改变运动信号通路关键蛋白P-JNK和Rac-1的表达来抑制乳腺癌MDA-MB-231细胞的迁移,且该作...

Objective：To study the impacts of Zhuanggu Zhentong Capsules（ZZC） on the cell migration and the expressions of key proteins in migrating-related signaling pathway such as p-JNK and Rac-1 of breast cancer cell lines MDA - MB -231 in vitro. Methods：The impacts of different doses of ZZC contained plasma （5.0%,1.25% and 0.63%） on the migrations of MDA - MB -231 cells in intro were measured by wound-healing assay. These proteins levels were assayed by immunocytochemistry protocol. Results： The cell migration and the expressive levels of p-J...