Introduction: Plasma cell-free DNA (cfDNA) of trauma patients has been widely investigated, but it has not been resolved whether cfDNA can be used as a non-invasive, rapid and sensitive marker of injury. Here we evaluated serum cfDNA in patients after injury and assessed the relationship between cfDNA levels and clinical prognosis. Methods: Fifty-six trauma patients formed three groups (minor, moderate and severe) according to Injury Severity Score (ISS), 33 of these cases were also divided according to microbiological and clinical evidence of infection. Plasma cfDNA and other indices were mea...