期刊论文

Effect of electroacupuncture at Neiguan(PC 6)and Baihui(GV 20)on CHOP and caspase-12 gene expressions in rats after ischemia-reperfusion injury

Ya-ping Lin

英文

针灸推拿医学：英文版

1672-3597

2017

15

1

8-13

10.1007/s11726-017-0967-1

supported by National Natural Science Foundation of China,No.81202771（国家自然科学基金,No.81202771）

本校为第一且通讯机构

Objective:To investigate the effects of electroacupuncture(EA)at Neiguan(PC 6)and Baihui(GV 20)by observing the changes of CCAAT/enhancer-binding protein(C/EBP)homologous protein(CHOP)and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury(IRI),and explore whether the apoptosis pathway of endoplasmic reticulum stress(ERS)is involved in the protective mechanisms of EA.Methods:Sixty rats were randomly assigned to five groups(12 in each group):a normal control group(group A),a sham-operation group(group B),an operation group(group C),an Edaravone group(group D)and an EA...

目的：通过观察CHOP和Caspase-12基因表达在脑缺血再灌注大鼠体内的变化探讨电针内关、百会的效应以及电针保护性机制是否与内质网应激介导的凋亡通路有关。方法：将60只大鼠随机分为5组,每组12只,即正常对照组(A)、假手术组(B)、模型组(C)、依达拉奉组(D)、电针干预组(E)。采用线栓法结扎大脑中动脉制备大鼠局灶性脑缺血再灌注模型,2,3,5-氯化三苯基四氮唑(TTC)染色测定脑梗死体积, RT-PCR法测定缺血侧顶叶大脑皮层CHOP、caspase-12 mRNA表达变化。结果：与正常组(A)和假手术组(B)比较,模型组(C)、依达拉奉组(D)和电针组(E)脑梗死体积百分比、CHOP、caspase-12 mRNA表达均显著增高(P＜0.01或P＜...