期刊论文

Effect of electroacupuncture on calcium-activated chloride channel currents in interstitial cells of Cajal in rats with diabetic gastroparesis

Zhang Cheng-cheng<&wdkj&>Peng Yan

英文

针灸推拿医学：英文版

1672-3597

2021

19

1

1-9

10.1007/s11726-021-1222-3

This work was supported by National Natural Science Foundation of China (国家自然科学基金项目, No. 81774431); Open Fund of the Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine (湖南中医药大学 中医学一流学科开放基金, No. 2018ZYX35).

本校为第一且通讯机构

Objective: To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Methods: Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2% streptozotocin (STZ) combined with 8-week high-glucose high-f...

目的:基于钙激活氯离子通道探讨电针足三里、梁门和三阴交治疗糖尿病胃轻瘫(DGP)的作用机制。方法:将40只Sprague-Dawley大鼠随机分为4组,即空白对照组(A组)、模型组(B组)、电针组(C组)和胃复安组(D组),每组10只。采用单次腹腔注射2%的链脲佐菌素(STZ)和8周高糖高脂饮食建立DGP大鼠模型。干预后测定胃肠推进率,免疫组化检测跨膜蛋白16A(TMEM16A)的表达水平,免疫荧光法检测Cajal间质细胞(ICCs)内Ca~(2+)浓度,应用全细胞膜片钳技术检测胃窦ICCs中钙激活氯离子通道电流强度(ICaCC)。结果:造模后, B、C和D组血糖较A组明显升高(均P