期刊论文

Simultaneous Determination of Three Active Components in Penyanjing by Ultra-high Performance Liquid Chromatography

中文

分析科学学报

1006-6144

2020

36

01

52-56

10.13526/j.issn.1006-6144.2020.01.010

No.2019JJ50446:湖南省自然科学基金青年项目 No.kq1801044:长沙市科技局项目 :湖南省中药饮片标准化 No.2018-02:功能工程技术研究中心开放基金 No.ZYBZH-Y-HUN-24:国家中医药局科研项目 No.2017-21:大学生创新项目

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建立了超高效液相色谱法(UPLC)同时检测中成药盆炎净胶囊、片剂、颗粒剂和咀嚼剂不同剂型中原儿茶酸、芍药苷和大车前苷3种有效成分的分析方法.采用AglientEclipse Phus C18色谱柱(50 mm×2.1 mm,1.8 nm),流动相为乙腈-0.2％磷酸溶液,梯度洗脱,10 min内可完成这3种有效成分的同时分离检测.原儿茶酸线性回归方程为:Y=19.187X+12.196(r=0.9998),线性范围0.34～34.00 μg/mL;芍药苷线性回归方程为:Y=29.946X+9.044(r=0.9997),线性范围0.57～57.14 μg/mL;大车前苷线性回归方程为:Y=31.633X+0.2233(r=0.9998),线性范围0.49～48.81 μg/ml.原儿茶酸、芍药苷和大车前苷的平均回收率分别为103.40％、98...

In this work,we established a method of ultra-high performance liquid chromatography (UPLC)for the simultaneous determination of 3active constituents in Penyanjing capsules,tablets, granules and chewables.Aglient-1290fully automatic UPLC was used to detect protocatechuic acid, paeoniflorin and plantamajoside simultaneously in 10 min.Gradient elution of acetonitrile-0.2% phosphoric acid aqueous solution was adopted at the flow rate of 0.15mL/min.The detection wavelength was 230nm,injection volume was 2.0μL,and the column temperature was maintai...