期刊论文

The Effect of Spleen- strengthening Therapy on the mRNA and protein expression levels of INTβ_3,ILK and FAK in Rats with Cerebral Ischemia /Reperfusion Injury

中文

时珍国医国药

1008-0805

2017

28

3

513-516

10.3969/j.issn.1008-0805.2017.03.001

No.81202632:国家自然科学基金 No.81473567:国家自然科学基金 No.20124323120003:高等学校博士学科点专项科研基金 No.13JJ3097:湖南省自然科学基金 No.14B134:湖南省教育厅优秀青年项目 No.15K092:湖南省教育厅高校创新平台基金项目

本校为第一机构

目的研究健脾补土法组方对脑缺血/再灌注损伤大鼠测定整合素β_3(INTβ_3)、ILK、FAK mRNA及蛋白的表达影响。方法将36只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组、健脾补土方小剂量组、健脾补土方中剂量组、健脾补土方大剂量组6小组,每组6只。采用线栓法制备MCAO模型,7天后处死,应用RT- PCR法测定缺血再灌注后缺血区脑皮质内INTβ_3、ILK、FAK mRNA的表达的含量变化及运用健脾补土法组方对其含量的影响,Western blot法检测其蛋白含量变化。结果模型组与假手术组相比,模型组INTβ_3、ILK、FAK蛋白以及mRNA含量显著减少(P < 0.01);与模型组比较,依达拉奉组、健脾补土小剂量、健脾补土中剂...

Objective To study the effect of spleen- strengthening therapy on the expression of INT,ILK and FAK in rats with cerebral ischemia /reperfusion injury. Methods 36 male SD rats were randomly divided into sham- operation group,modelling group,edaravone group and,low- dosed spleen- strengthening group,middle- dosed spleen- strengthening group,high- dosed spleen- strengthening group,6 in each group. The model of MCAO was induced by middle cerebral artery occlusion and recanalization,and execute them after treated 7 days. RT- PCR was used to detect ...