期刊论文

中文

河北中医

1002-2619

2012

34

10

1540-1543

10.3969/j.issn.1002-2619.2012.10.058

2009年度广西中医药管理局中医药科技专项课题（编号:GZKZ09-21）；

本校为第一机构

目的 观察癌痛消颗粒对移植性肝癌模型大鼠肝癌及癌旁组织微小核糖核酸(miRNAs)表达的调节作用,探讨癌痛消颗粒抗肝癌作用的机制.方法 将60只移植性肝癌模型大鼠随机分为5组,即癌痛消颗粒高、中、低剂量组及5-氟尿嘧啶(5-Fu)对照组、蒸馏水对照组,另设10只大鼠为空白对照组,不进行造模及给药.于造模后第8d对癌痛消颗粒高、中、低剂量及蒸馏水对照组灌胃给药治疗,连续用药14 d后停药；5-Fu对照组腹腔注射给药治疗,共连续注射5次,各组均停药24h后处死各组大鼠,取出瘤块,用实时定量PCR(PT-PCR)技术观察肝癌及癌旁组织特定miRNAs中miR-199a-3p、miR-199a-5p、miR-18表达水平.结果 癌痛消颗粒高、中、...

Objective To observe the regulation function of Aitongxiao granule on miRNAs expression of hepatocellular carcinoma and adjacent tissue in transplanted liver cancer rat model. Methods 60 transplanted liver cancer model rats were randomly divided into five groups, high - , middle - , low - dose Aitongxiao granule group, 5 - Fu control group and distiller water group. Another 10 rats were used as a blank control group, without the model- ing and administration. At 8th day after modeling rats in high - , middle - , low - dose Aitongxiao granule gr...