期刊论文

中文

中成药

1001-1528

2014

36

6

1203-1208

10.3969/j.issn.1001-1528.2014.06.020

本校为第一机构

目的建立同时测定荆防小儿止咳口服液(荆芥、防风、连翘和苦杏仁等)中升麻素苷和连翘苷的方法。方法以升麻素苷和连翘苷的量为评价指标,选择氧化铝柱和洗脱剂种类和用量。色谱条件采用Kromasil ODS1(250 mm × 4.6 mm,5 μm)色谱柱,以乙腈-水为流动相进行梯度洗脱(0 ~ 15 min,16% 乙腈; 15 ~ 50 min,22% 乙腈),体积流量1.0 mL/min,检测波长277 nm,柱温30 ℃。结果氧化铝柱层析条件是用中性氧化铝干法装柱,洗脱剂为50%乙醇,洗脱体积为40 mL。升麻素苷在0.093 6 ~ 0.702 0 μg 范围内线性良好(r=0.999 8),平均回收率为98.88%,RSD 为2.18%(n=6); 连翘苷在0.110 4 ~ 0.828 0 μg 范围内线性良好(r=0...

AIM To establish a method for simultaneously determining the contents of prim-O-glucosylcimifu- gin and forsythin in Jingfang Children Cough Oral Liquid （Schizonepetae Herba, Saposhnikoviae Radix, Forsythiae Fructus, AmeniacaeSemen amarum, etc）. METHODS The choices of Al2O3 column chromatograph and elution solvent were made among the type and use level with the content of prim-O-glucosylimfugin and forsythin as evalua- tion indexes. The HPLC analysis of samples was carried out on RP-C18 column （250 mm ×4. 6 mm, 5 μm） with acetonitrile -wa...