期刊论文

Effects of Bushen Huoxue Recipe on the expressions of TGF-β1,TGF-βRII and Smad2 /3 in granulosa cells in mice with premature ovarian failure

中文

中成药

1001-1528

2017

39

9

1782-1788

10.3969/j.issn.1001-1528.2017.09.005

81303123:国家自然科学基金 15K090:湖南省教育厅创新平台项目 2014XNFZ06:湖南省重点实验室开放基金 2016-281:湖南省大学生创新项目 201510541002:大学生创新项目

本校为第一机构

目的探索补肾活血方(紫石英、补骨脂、菟丝子等)对免疫性卵巢早衰(POF)小鼠卵泡壁颗粒细胞转化生长因子(TGF)-β1、TGF-βRII、Smad2 /3蛋白表达的影响。方法Balb /c雌性小鼠皮下多点注射小鼠透明带3建立免疫性POF模型,POF小鼠分成模型组,阳性组(戊酸雌二醇)和补肾活血方低、中、高剂量组。干预30 d后,卵巢组织作苏木精-伊红(HE)染色,免疫组化法与蛋白免疫印迹法(Western blot)分别检测卵泡壁颗粒细胞和卵巢组织TGF-β1、TGF-βRII、Smad2 /3的蛋白表达。结果与模型组相比,补肾活血方各剂量组、阳性组卵巢成熟卵泡数目明显增多,闭锁卵泡数目减少。卵泡壁颗粒细胞和卵巢组织TGF-β1、TGF-βRII、...

AIM To explore the effects of Bushen Huoxue Recipe (Fluoritum,Psoraleae Fructus,Cuscutae Semen,etc.) on the protein expressions of transforming growth factor (TGF)-β1,TGF-βRII and Smad2 /3 in follicle wall granulosa cells in mice with autoimmune premature ovarian failure (POF). METHODS Balb /c female mice were subcutaneously injected with mouse zona pellucida 3 at multiple points to establish autoimmune POF model. POF mice were divided into model group,positive group (progynova),low-,middle-and high-dose of Bushen Huoxue Recipe groups. After ...