期刊论文

Establishment of HPLC fingerprints of Panax japonicus and determination of seven constituents

中文

中成药

1001-1528

2019

41

5

1074-1080

10.3969/j.issn.1001-1528.2019.05.023

湖南省自然科学基金面上项目(2019JJ40172) 湖南省中医药科研计划重点项目(201811) 长沙市科技计划重点项目(kq1801034) 湖南中医药大学一层次学科中药学资助(湘教通[2011]76).

本校为第一机构

目的 建立竹节参Panax japonicusC.A.Mey HPLC指纹图谱,并测定7种核苷类成分的含有量.方法 竹节参0.1％氨水提取液的分析采用Venusil MP C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.01％甲酸水,梯度洗脱;体积流量0.8 mL/min;柱温30℃;检测波长254 nm.对结果 进行聚类分析和主成分分析.结果 10批样品指纹图谱中有16个共有峰,除S2外相似度均在0.90以上.7种成分在各自范围内线性关系良好(R2≥0.999 6),平均加样回收率92.74％～98.66％,RSD 2.32％～2.97％.10批样品分为3类.结论 该方法 简便可靠,重复性好,可用于竹节参药材的质量控制.

AIM To establish the HPLC fingerprints of Panax japonicus C.A.Mey and to determine the contents of seven nucleosides constituents.METHODS The analysis of 0.1% ammonia aqueous extract of P.japonicus was performed on a 30 ℃ thermostastic Venusil MP C_(18) column (250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.01% formic acid flowing at 0.8 mL /min in a gradient elution manner,and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were s...