期刊论文

Chemical constituents from the flowers of Acanthopanax henryi and their antiinflammatory activities

中文

中成药

1001-1528

2019

41

8

1856-1862

10.3969/j.issn.1001-1528.2019.08.021

湖南省自然科学基金项目(11JJ2042) 湖南中医药大学生物工程重点学科资助([2018]3).

本校为第一机构

目的研究糙叶五加Acanthopanax henryi( Oliv.) Harms花的化学成分及其粗提物抗炎活性。方法糙叶五加花70%甲醇提取物乙酸乙酯部位采用正相硅胶、反相C_(18)柱、Sephadex LH-20、HPLC、制备薄层和重结晶等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用脂多糖( LPS)诱导的巨噬细胞RAW264.7和小胶质细胞BV2为筛选模型评价提取物抗炎活性。结果从中分离得到17个化合物,分别鉴定为槲皮素-3-O-β-D-吡喃葡萄糖苷( 1) 、山柰酚-3-O-β-D-吡喃葡萄糖苷( 2) 、山柰酚-3-O-α-L-鼠李糖苷( 3) 、山柰酚-3-芸香糖苷( 4) 、1,5-双咖啡酰奎宁酸( 5) 、1,4-双咖啡酰奎宁酸( 6) 、1,3-双咖啡...

AIM To study the chemical constituents from the flowers of Acanthopanax henryi ( Oliv.) Harms and the anti-inflammatories activities of the extract. METHODS The ethyl acetate fraction of 70% methanol ex- tract from A. henryi was isolated and purified by NP-silica,RP-C18,Sephadex LH-20,HPLC,Prep-TLC and re- crystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data. Their anti-inflammatory activities of the extract were estimated by LPS-induced inflammatory model in RAW264. 7 macroph...